Journal: iScience

Article Title: Changes in the microglial phenotype drive neuroinflammation independent of systemic inflammation in the acute stage of heatstroke

doi: 10.1016/j.isci.2026.115254

Figure Lengend Snippet: Time course of functional damage to the intestinal barrier and blood-brain barrier during the acute stage of heatstroke The mice were exposed to 41.2 ± 0.5°C ambient temperature until their rectal temperature reached 42.4°C, and then they were allowed to recover at an ambient temperature of 25 ± 0.5°C for the indicated times. (A) The concentration of FD-4 in serum was measured after its oral administration. (B) Serum endotoxin levels. (C) EB was injected into the mice via the tail vein after the onset of heatstroke, and representative images of EB leakage was shown. (D) Quantitative analysis of EB concentrations in brain tissues. (E) Expression of MMP-2 in the cerebral cortex at 1 h, 6 h, and 24 h post heatstroke. (F) Expression of MMP-9 in the cerebral cortex at 1 h, 6 h, and 24 h post heatstroke. The data are expressed as the mean ± SEM of three independent experiments. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, vs. Con.

Article Snippet: The membrane was further incubated overnight at 4°C with primary antibodies against MMP9 (TA326652; ORIGENE, USA), MMP2 (TA326260; ORIGENE, USA) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 2118L; CST, USA).

Techniques: Functional Assay, Concentration Assay, Injection, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Suppressive Potential of Ethanolic Extracts of Parkia speciosa Hassk. Empty Pods Against Colon Cancer Cell Migration and Invasion

doi: 10.3390/ijms27042072

Figure Lengend Snippet: Western blot showing the effects of the PSET on the abundance of proteins associated with the downstream EMT pathway. Representative Western blot image showing the amount of MMP2, MMP9 and GAPDH proteins in control (untreated cells) and treatment (PSET) groups. The molecular weight (MW) of each protein is provided. Quantitative analysis of protein levels following Western blot analysis. Data in histograms are presented as the means ± SDs from three independent experiments ( n = 3), with asterisks indicating statistical differences: * p < 0.05.

Article Snippet: The primary antibodies used were rabbit polyclonal anti-human MMP2 (#4022), MMP9 (#3852), N-cadherin (#4061), and GAPDH (#2118) (dilution 1:500; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Western Blot, Control, Molecular Weight